Coding

Part:BBa_M33335:Design

Designed by: Christopher Brunson   Group: Stanford BIOE44 - S010   (2010-06-05)

CDS for Inorganic mercury-sensitive regulatory protein merR coding sequence with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 221
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to add a medium strength RBS (AAAGAGGAGAAA)


Source

Thermus thermophilus HB27, complete genome GenBank: AE017221.1 773178..773606

References

An initial characterization of the mercury resistance (mer) system of the thermophilic bacterium Thermus thermophilus HB27. Wang Y, Freedman Z, Lu-Irving P, Kaletsky R, Barkay T. FEMS Microbiol Ecol. 2009 Jan;67(1):118-29. Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA.